Intermediate

Part:BBa_K300983:Design

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-09)

BBa_K300000 construction intermediate (provided by Mr Gene)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 635
    Illegal XbaI site found at 24
    Illegal XbaI site found at 805
    Illegal SpeI site found at 212
    Illegal PstI site found at 652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 635
    Illegal NheI site found at 43
    Illegal NheI site found at 459
    Illegal SpeI site found at 212
    Illegal PstI site found at 652
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 635
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 635
    Illegal XbaI site found at 24
    Illegal XbaI site found at 805
    Illegal SpeI site found at 212
    Illegal PstI site found at 652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 635
    Illegal XbaI site found at 24
    Illegal XbaI site found at 805
    Illegal SpeI site found at 212
    Illegal PstI site found at 652
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See BBa_K300000 for details about the design of this part. BBa_B0033 was inserted between the EcoRI and PstI restriction sites to provide a spacer between the two sites. In the BBa_K300000 assembly process, BBa_B0033 was eliminated.

Source

Mr Gene synthesis service (www.mrgene.com).

References

See BBa_K300000.